Idizayini yokuhlola
Izinto zokuhlola: ubisi olusha,i-xylo-oligosaccharides, Lactobacillus bulgaricus (Lb), Streptococcus thermophilus (St).
Ukwenza kusebenze i-Strain: Yenza kusebenze i-Lactobacillus bulgaricus ne-Streptococcus thermophilus obisini lwe-skim izikhathi ezintathu kuze kube yilapho umsebenzi wazo uzinzile (okwahlulelwa ngokuhlolwa kwe-microscopic kanye nobisi olugobile), bese uzigcina esiqandisini ukuze zisetshenziswe kamuva. Indlela yokuhlola: Lungiselela amanani alinganayo angama-21 amasampula obisi olusha bese uwahlukanisa abe amaqembu ama-3, namasampula ayi-7 eqenjini ngalinye. Engeza ukugxilisa ingqondo (%, w/v) kokungu-0, 0.5, 0.6, 0.7, 0.8 kumasampula eqembu ngalinye. , 0.9 kanye no-1.0 xylo-oligosaccharides, bese uxuba i-Lb ne-St ngokulingana kuka-2:1, 1:1, no-1:2 ngokulandelana ukuze wenze isiko lokuqala, bese ujova emaqenjini angu-3 ngenani lokujova lika-3%. Bilisa ekushiseni okungashintshi okungama-42°C, futhi uthathe amasampula ngezikhathi ezithile ukuze ulinganise inani lamagciwane asebenzayo, inani le-pH, i-asidi kanye nokuqukethwe kwe-lactic acid eqenjini ngalinye lobisi.
imiphumela yokuhlolwa
(1) Lb: St = 2: 1, umphumela we-xylo-oligosaccharides ekukhuleni nasekukhiqizeni i-asidi yezinhlobo zokuvutshelwa kweyogathi
Njengoba kungabonwa kuMfanekiso 1, i-0 kuya ku-1.5 h isikhathi sokuzivumelanisa nokucindezeleka kanye nesigaba sokuqala sokungena esigabeni sokukhula kwe-logarithmic. Umuthi awukhuli ngokushesha, inani le-pH lehla kancane, futhi i-asidi ne-lactic acid ikhula ngokushesha. Lapho ukuhlushwa kwe-xylo-oligosaccharide kungu-0.7%, isamba sebhaktheriya Inani eliphakeme ngu-1.94×108 CFU/mL (ukulawula kungu-1.15×108 CFU/mL), kanye ne-pH, i-asidi ne-lactic acid okuqukethwe kuyafana nokulawula; phakathi kuka-1.5 h no-3.5 h, ubunzima bungena esigabeni sokukhula kwe-logarithmic futhi lukhula ngokushesha. I-pH yehla ngokushesha, futhi i-asidi ne-lactic acid okuqukethwe kwanda ngokushesha. Lapho ukuhlushwa kwe-xylo-oligosaccharide kungu-0.7%, inani eliphezulu lenani lebhaktheriya lalingu-5.65.×108 CFU/mL (ukulawula kwakungu-5.41×108 CFU/mL). I-pH, i-asidi, ne-lactic acid okuqukethwe Kwakuyi-5.82, 63 kanye no-4.93 ngokulandelanayo, futhi i-pH, i-asidi ne-lactic acid okuqukethwe kokulawula kube ngu-6.05, 56 kanye no-4.67. Kusukela emahoreni angu-3.5 kuya ku-4.5, inani lebhaktheriya liyancipha, i-pH yehla kancane, futhi i-acidity ne-lactic acid okuqukethwe kwenyuka kancane.
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Isikhathi sokuthumela: Nov-14-2023
